The SARS coronavirus E protein interacts with PALS1 and alters

1 2 3 4 The SARS coronavirus E protein interacts with PALS1 and alters 5 tight junction formation and epithelial morphogenesis 6 7 8 9 10 11 Kim-Tat Teoh, Yu-Lam Siu, Wing-Lim Chan, Marc A. Schlüter, Chia-Jen Liu, J. S. 12 Malik Peiris, Roberto Bruzzone, Benjamin Margolis, and Béatrice Nal 13 14 15 16 HKU-Pasteur Research Centre, 8 Sassoon Road, Pokfulam, Hong Kong S.A.R. China, 17 Department of Paediatrics and Adolescent Medicine, Department of Pathology, Department of 18 Microbiology, Department of Anatomy, 21 Sassoon Road, The Li Ka Shing Faculty of 19 Medicine, The University of Hong Kong, Hong Kong S.A.R., China, 20 Medizinische Klinik D, Universitätsklinikum Münster, Albert-Schweitzer-Str. 33, D-48149 21 Münster, Germany, 22 Department of Internal Medicine and Biological Chemistry, University of Michigan Medical 23 School, 109 Zina Pitcher Place, Ann Arbor, MI 48109 24 25 ^These two authors equally contributed to this work. 26 27 Correspondence to: Béatrice Nal, HKU-Pasteur Research Centre, 8 Sassoon Road, Pokfulam, 28 Hong Kong SAR, China. Tel: +852-2816 8422 / Fax: +852-2872 5782; e-mail: [email protected] 29 30 31 32 33 34 Running Head: SARS-CoV E alters epithelium integrity (34 characters) 35 36 37 38 Abbreviations: AJ, adherens junction; CRB3, CRUMBS3; PALS1, Protein associated with Lin39 Seven 1; PATJ, PALS1 associated tight junction; TJ, tight junction; PBM, PDZ domain-binding 40 motif, PDZ, Post-synaptic density protein-95/Discs Large/Zonula occludens-1; SARS-CoV, 41 Severe acute respiratory syndrome-coronavirus. 42 43